Bucculite(TM) Flow Cytometric XdU Cell Proliferation Assay Kit *Violet Laser-Compatible*

Monitoring cell proliferation is one of the most reliable methods to assess cell viability, cell cycles and genotoxicity. An essential way to detect cell proliferation is to measure DNA synthesis in the presence of thymidine during the S-phase of cells growth. Bucculite(TM) Flow Cytometric XdU Cell Proliferation Assay Kit uses XdU which is incorporated into cellular DNA during DNA synthesis. After fixing cells, the incorporated XdU is labelled with mFluor(TM) Violet 450 azide. The resulted mFluor(TM) Violet 450-labeled DNA formed in cells is visualized in Pacific Blue Channel. Bucculite(TM) Flow Cytometric XdU Cell Proliferation Assay Kit provides an alternative to anti-BrdU antibody-based assay and EdU click chemistry assay. It is sensitive and might be used for measuring active DNA synthesis at single-cell level.