Cell Meter(TM) Cellular Senescence Activity Assay Kit *Red Fluorescence*

Cellular Senescence is an irreversible growth arrest triggered in order to prevent growth in DNA damaged cells. Senescence-associated beta-galactosidase is highly overexpressed in senescent cells and has been widely used as a senescence marker. The colorimetric X-gal staining method is widely used to detect SA-beta-gal in senescent cells. However, the color method has some limitations such as the requirement of fixation of cells (due to the low cell permeability of X-gal), longer staining time and low sensitivity. Cell Meter(TM) Cellular Senescence Activity Assay Kit uses Xite(TM) Red beta-D-galactopyranoside, a fluorogenic beta-Gal substrate that readily enters into live cells, and gets cleaved by beta-galactosidase inside cells, generating strong red fluorescence. Unlike cell-impermeable X-Gal substrate, it has excellent cell permeability. The robust Cell Meter(TM) Cellular Senescence Activity Assay Kit enables users to detect the senescence with higher sensitivity. Xite(TM) Red beta-D-galactopyranoside is fixable for further cell analysis if desired. The red fluorescence of Xite(TM) Red can be readily combined with other color fluorescent probes such as DAPI or GFP. The Xite Red product is well retained inside cells, producing a stable signal for fluorescence imaging and flow cytometry analysis.