FLICA(TM) 660 Poly Caspases Assay Kit,far red-fluorescence (25-50 tests)

The red FLICA® 660-VAD-FMK caspase detection probe is comprised of the preferred affinity peptide sequence (VAD) targeted by activated pan-caspases, a far-red fluorescent 660 dye label, and a fluoromethyl ketone (FMK) reactive moiety. The resulting fluorescent caspase inhibitor probe forms an irreversible, covalent bond with active caspase enzymes, efficiently labeling the target for detection. Due to its cell permeant nature and fluorescence properties, the far-red FLICA poly caspase probe enables whole cell analysis via common fluorescence detection methods. To use FLICA, add it directly to suspension cell or tissue culture media, incubate, and wash. The cell permeant FLICA 660-VAD-FMK reagent will efficiently diffuse into cells and irreversibly bind to activated caspase enzymes, thereby retaining the red signal inside caspase-positive cells. Cells not bearing active caspases return to a non-fluorescent status after the wash step. The FLICA® 660 poly caspase probe has an optimal excitation at 660 nm and optimal emission range from 680-690 nm. As such, it has demonstrated excellent excitation efficiency with a conventional red HeNe laser with a 633 nm excitation, enabling samples to be analyzed with most flow cytometers and fluorescence microscopes equipped with electronic grey scale image capabilities. Cells labeled with the FLICA® reagent may be read immediately or preserved for 16 hours using the fixative.