Cell Meter(TM) Live Cell Caspase 3/7 Binding Assay Kit *Green Fluorescence*

Our Cell Meter(TM) live cell caspases activity assay kits are based on fluorescent FMK inhibitors of caspases. These inhibitors are cell permeable and non-cytotoxic. Once inside the cell, the caspase inhibitors bind covalently to the active caspases. The activation of caspase 3/7 is important for the initiation of apoptosis. It has been proven that caspase 3/7 has substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). This kit uses FAM-DEVD-FMK as a fluorescent indicator for caspase 3/7 activity. FAM-DEVD-FMK irreversibly binds to activated caspase 3/7 in apoptotic cells. Once bound to caspase 3/7, the fluorescent reagent is retained inside the cell. The binding event inhibits caspase 3/7 but will not stop apoptosis from proceeding. There are a variety of parameters that can be used for monitoring cell apoptosis. This Cell Meter(TM) Live Cell Caspase 3/7 Activity Assay Kit is designed to detect cell apoptosis by measuring caspase 3/7 activation in live cells. It is used for the quantification of activated caspase 3/7 activities in apoptotic cells, or for screening caspase 3/7 inhibitors. FAM-DEVD-FMK, the green label reagent, allows for direct detection of activated caspase 3/7 in apoptotic cells by fluorescence microscopy, flow cytometer, or fluorescent microplate reader. The kit provides all the essential components with an optimized assay protocol.