XCL1/Lymphotactin, BioAssay(TM) ELISA Kit (Mouse)

Chemokine(C motif) ligand(XCL1) is a small cytokine belonging to the XC chemokine family that is also known as lymphotactin. This gene is mapped to chromosome 1q23 by FISH. The sequence of the deduced 114aa protein is most homologous to the CC chemokines CCL8 and CCL3. It is found in high levels in spleen, thymus, intestine and peripheral blood leukocytes, and at lower levels in lung, prostate gland and ovary. XCL1 induces the migration of cells expressing XCR1. Sample Type: Cell culture supernates, serum and plasma (heparin, EDTA), Intended Use: For quantitative detection of mouse XCL1 in cell culture supernates, serum and plasma (heparin, EDTA). Sensitivity: <5pg/ml, Range: 62.5-4000pg/ml, Specificity: Natural and recombinant mouse XCL1, Test Principle: This mouse XCL1 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for XCL1 has been precoated onto 96-well plates. Standards (E. coli) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for XCL1is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse XCL1 amount of sample captured in plate. Kit Components: 96-well plate precoated with mouse XCL1antibody, 1, Lyophilized recombinant mouse XCL1 standard, 10ng/tube×2, Biotinylated mouse XCL1 antibody, 130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100), Sample diluent buffer, 30ml, Antibody diluent buffer, 12ml, ABC diluent buffer, 12ml, TMB color developing agent, 10ml, TMB stop solution, 10ml, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.