TGF-Beta 2 BioAssay(TM) ELISA Kit (Bovine)

TGF-Beta 2 BioAssay(TM) ELISA Kit (Bovine)
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
396774.96 96 tests - -

3 - 19 Werktage*

947,00 €
 
Transforming growth factor-beta 2(TGF-beta 2) is a secreted protein known as a cytokine that... mehr
Produktinformationen "TGF-Beta 2 BioAssay(TM) ELISA Kit (Bovine)"
Transforming growth factor-beta 2(TGF-beta 2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. This gene is mapped to 1q41. It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. TGF-beta 2 is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma(POAG). Studies have shown that TGF-beta 2 influences cultured trabecular meshwork cells, and it reduced outflow facility when perfused into cultured Bovine anterior segments. In POAG, elevated expression of Gremlin by TM cells inhibited BMP4 antagonism of TGF-beta 2 and led to increased extracellular matrix deposition and elevated IOP. Sample Type: Cell culture supernates, serum, plasma (heparin or EDTA). Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Bovine TGF-beta 2. Sensitivity: <10pg/ml, Range: 31.2pg/ml-2000pg/ml, Specificity: Recognizes natural and recombinant Bovine TGF-beta 2. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Bovine TGF-Beta 2 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Bovine TGF-Beta 2 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: A303-S414) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Bovine TGF-Beta 2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Bovine TGF-Beta 2 amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Bovine TGF-beta 2 antibody, 1x plate, Lyophilized recombinant Bovine TGF-beta 2 standard, 2x10ng/tube, Biotinylated anti-Bovine TGF-beta 2 antibody, 1x130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100), Sample diluent buffer, 1x30ml, Antibody diluent buffer, 1x12ml, ABC diluent buffer, 1x12ml, TMB color developing agent, 1x10ml, TMB stop solution, 1x10ml, PBS washing buffer Powder for 1000ml, 1x buffer, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Schlagworte: TGFB2
Hersteller: United States Biological
Hersteller-Nr: 396774

Eigenschaften

Anwendung: ELISA
Wirt: Bovine
Spezies-Reaktivität: bovine
Format: Solid Phase

Handhabung & Sicherheit

Lagerung: vT
Versand: +4°C (International: °C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
Hier kriegen Sie ein Zertifikat
oder , um Analysenzertifikate anzufordern.
Bewertungen lesen, schreiben und diskutieren... mehr
Kundenbewertungen für "TGF-Beta 2 BioAssay(TM) ELISA Kit (Bovine)"
Bewertung schreiben
oder , um eine Produktbewertung abzugeben.
Zuletzt angesehen