Semaphorin 7A (SEMA7A) BioAssay(TM) ELISA Kit (Mouse)

Semaphorin 7A (SEMA7A) BioAssay(TM) ELISA Kit (Mouse)
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
028027.96 96 tests - -

3 - 19 Werktage*

833,00 €
 
Semaphorin 7A (SEMA7A) BioAssayT ELISA Kit (Mouse) is a sandwich enzyme immunoassay for in vitro... mehr
Produktinformationen "Semaphorin 7A (SEMA7A) BioAssay(TM) ELISA Kit (Mouse)"
Semaphorin 7A (SEMA7A) BioAssayT ELISA Kit (Mouse) is a sandwich enzyme immunoassay for in vitro quantitative measurement of SEMA7A in mouse serum, plasma, tissue homogenates, cell lysates and other biological fluids. Detection Range: 0.312-20ng/ml, Sensitivity: 0.114ng/ml, Intra-Assay CV: < 10%, Inter-Assay CV: < 12%, Kit Components: *028027A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use, *028027B: Standard, 2x1 vial, 028027C: Standard Diluent, 1x20ml, *028027D: Detection Reagent A, 1x120ul, *028027E: Detection Reagent B, 1x120ul, 028027F: Assay Diluent A, 1x12ml, 028027G: Assay Diluent B, 1x12ml, 028027H: TMB Substrate, 1x9ml, 028027K: Stop Solution, 1x6ml, 028027L: Wash Buffer, 30X, 1x20ml, Precaution: The Stop Solution (028027K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *028027A, *028027B, *028027D and *028027E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards,, 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C., 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C., 4. Aspirate and wash 3 times., 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C., 6. Aspirate and wash 5 times., 7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C., 8. Add 50ul Stop Solution. Read at 450nm immediately.
Schlagworte: CD108, Cd108, Sema L, Sema7a, Sema K1, Semaphorin-L, Semaphorin-7A, Semaphorin-K1
Hersteller: United States Biological
Hersteller-Nr: 028027

Eigenschaften

Anwendung: ELISA
Reaktivität: Mouse
Format: Solid Phase

Handhabung & Sicherheit

Lagerung: vT
Versand: +4°C (International: +4°C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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