Relaxin 2 BioAssay(TM) ELISA Kit (Human)

Relaxin 2 BioAssay(TM) ELISA Kit (Human)
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
396750.96 96 tests - -

3 - 19 Werktage*

947,00 €
 
Relaxins are known endocrine and autocrine/paracrine hormones, belonging to the insulin gene... mehr
Produktinformationen "Relaxin 2 BioAssay(TM) ELISA Kit (Human)"
Relaxins are known endocrine and autocrine/paracrine hormones, belonging to the insulin gene superfamily. In humans there are three non-allelic relaxin genes, RLN1, RLN2 and RLN3, where RLN1 and RLN2 share high sequence homology. The active form of the protein encoded by this gene consists of an A chain and a B chain linked by disulfide bonds. Relaxin is produced by the ovary, and targets the mammalian reproductive system to ripen the cervix, elongate the pubic symphysis and inhibit uterine contraction. It may have additional roles in enhancing sperm motility, regulating blood pressure, controlling heart rate and releasing oxytocin and vasopressin. Relaxin 2(RLN2) is a polypeptide hormone structurally related to insulin and insulin-like growth factors (IGFs). However it does not interact with insulin receptors and has decidedly different biological properties. Relaxin 2 activates LGR 7 and LGR 8 relaxin receptors, that belong to the leucine-rich repeat-containing (LRR), G protein-coupled receptors. Sample Type: Cell lysates, cell culture supernates, serum, plasma (heparin or EDTA). Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Human Relaxin 2. Sensitivity: <4pg/ml, Range: 7.8pg/ml-500pg/ml, Specificity: Recognizes natural and recombinant Human Relaxin 2. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Human Relaxin 2 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A specific for Relaxin 2 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: Q162-C185(A)&D25-S53(B)) and test samples are added to the wells, a biotinylated detection specific for Relaxin 2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Relaxin 2 amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Human RLN2 antibody, 1x plate, Lyophilized recombinant Human RLN2 standard, 2x10ng/tube, Biotinylated anti-Human RLN2 antibody, 1x130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100), Sample diluent buffer, 1x30ml, Antibody diluent buffer, 1x12ml, ABC diluent buffer, 1x12ml, TMB color developing agent, 1x10ml, TMB stop solution, 1x10ml, PBS washing buffer Powder for 1000ml, 1x buffer, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Schlagworte: RLN2
Hersteller: United States Biological
Hersteller-Nr: 396750

Eigenschaften

Anwendung: ELISA
Wirt: Human
Spezies-Reaktivität: human
Format: Solid Phase

Handhabung & Sicherheit

Lagerung: vT
Versand: +4°C (International: °C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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