MBP-C/MBL2, BioAssay(TM) ELISA Kit (Human) (Mannose Binding Protein C)

MBP-C/MBL2, BioAssay(TM) ELISA Kit (Human) (Mannose Binding Protein C)
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
352970.96 96 tests - -

3 - 19 Werktage*

947,00 €
 
MBL2, also called mannose-binding lectin (protein C) 2, soluble or Mannose-binding lectin (MBL)... mehr
Produktinformationen "MBP-C/MBL2, BioAssay(TM) ELISA Kit (Human) (Mannose Binding Protein C)"
MBL2, also called mannose-binding lectin (protein C) 2, soluble or Mannose-binding lectin (MBL) is a lectin that is instrumental in innate immunity. MBL2 is mapped to chromosome 10q11.2-q21. It belongs to the class of collectins in the C-type lectin superfamily, whose function appears to be pattern recognition in the first line of defense in the pre-immune host. MBL2 recognizes carbohydrate patterns, found on the surface of a large number of pathogenic micro-organisms, including bacteria, viruses, protozoa and fungi. Binding MBL2 to a micro-organism results in activation of the lectin pathway of the complement system. Another important function of MBL2 is that this molecule binds senescent and apoptotic cells and enhances engulfment of whole, intact apoptotic cells, as well as cell debris by phagocytes. Sample Type: Cell culture supernates, serum and plasma (heparin, EDTA), Intended Use: For quantitative detection of human MBP-C in cell culture supernates, serum and plasma (heparin, EDTA). Sensitivity: <10pg/ml, Range: 312-20000pg/ml, Specificity: Natural and recombinant human MBP-C, Test Principle: This human MBP-C BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MBP-C has been precoated onto 96-well plates. Standards(NSO) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MBP-C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MBP-C amount of sample captured in plate. Kit Components: 96-well plate precoated with human MBP-C antibody, 1, Lyophilized recombinant human MBP-C standard, 20ng/tube×2, Biotinylated human MBP-C antibody, 130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100), Sample diluent buffer, 30ml, Antibody diluent buffer, 12ml, ABC diluent buffer, 12ml, TMB color developing agent, 10ml, TMB stop solution, 10ml, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Schlagworte: MBP1, MBL2, MBP-C, COLEC1, Collectin-1, Mannose-binding lectin, Mannan-binding protein, Mannose-binding protein C
Hersteller: United States Biological
Hersteller-Nr: 352970

Eigenschaften

Anwendung: ELISA
Wirt: Human
Spezies-Reaktivität: human
Format: Solid Phase

Handhabung & Sicherheit

Lagerung: vT
Versand: +4°C (International: °C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
Hier kriegen Sie ein Zertifikat
oder , um Analysenzertifikate anzufordern.
Bewertungen lesen, schreiben und diskutieren... mehr
Kundenbewertungen für "MBP-C/MBL2, BioAssay(TM) ELISA Kit (Human) (Mannose Binding Protein C)"
Bewertung schreiben
oder , um eine Produktbewertung abzugeben.
Zuletzt angesehen