Mannose Binding Lectin (MBL) BioAssay(TM) ELISA Kit (Human)

Mannose Binding Lectin (MBL) BioAssay(TM) ELISA Kit (Human)
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
026668.96 96 tests - -

1 - 19 Werktage

683,00 €
 
Mannose Binding Lectin (MBL) BioAssay(TM) ELISA Kit is a sandwich enzyme immunoassay for in vitro... mehr
Produktinformationen "Mannose Binding Lectin (MBL) BioAssay(TM) ELISA Kit (Human)"
Mannose Binding Lectin (MBL) BioAssay(TM) ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MBL in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Detection Range: 0.156-10ng/ml, Sensitivity: 0.069ng/ml, Intra-Assay CV: <10%, Inter-Assay CV: <12%, Kit Components: *026668A: Microtiter Strips, 1x48 wells or 1x96 wells, Pre-coated, ready to use, *026668B: Standard, 2x1 vial, 026668C: Standard Diluent, 1x10ml or 1x20ml, *026668D: Detection Reagent A (green), 1x60ul or 1x120ul, *026668E: Detection Reagent B (red), 1x60ul or 1x120ul, 026668F: Assay Diluent A, 1x6ml or 1x12ml, 026668G: Assay Diluent B, 1x6ml or 1x12ml, 026668H: TMB Substrate, 1x4.5ml or 1x9ml, 026668K: Stop Solution, 1x3ml or 1x6ml, 026668L: Wash Buffer, 30X, 1x10ml or 1x20ml, Precaution: The Stop Solution (026668K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *026668A, *026668B, *026668D and *026668E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards., 2. Add 100ul standard or sample to each well. Incubate 1 hour at 37°C., 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C., 4. Aspirate and wash 3 times., 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C., 6. Aspirate and wash 5 times., 7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C., 8. Add 50ul Stop Solution. Read at 450nm immediately.
Schlagworte: MBP1, MBL2, MBP-C, COLEC1, Collectin-1, Mannan-binding protein, Mannose-binding lectin, Mannose-binding protein C
Hersteller: United States Biological
Hersteller-Nr: 026668

Eigenschaften

Anwendung: ELISA
Reaktivität: Human
Format: Solid Phase

Handhabung & Sicherheit

Lagerung: vT
Versand: +4°C (International: +4°C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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