IL27 (human) SuperSet ELISA Kit

IL27 (human) SuperSet ELISA Kit
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Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
G-HUDC0129-1 96 wells (1 x 96 assays) - - -

9 - 20 Werktage

104,00 €
G-HUDC0129-5 480 wells (5 x 96 assays) - - -

9 - 20 Werktage

366,00 €
G-HUDC0129-10 960 wells (10 x 96 assays) - - -

9 - 20 Werktage

482,00 €
G-HUDC0129-15 1440 wells (15 x 96 assays) - - -

9 - 20 Werktage

566,00 €
G-HUDC0129-20 1920 wells (20 x 96 assays) - - -

9 - 20 Werktage

692,00 €
SuperSet ELISA Kits are a range of specially designed ELISA Assays with the pharmaceutical and... mehr
Produktinformationen "IL27 (human) SuperSet ELISA Kit"
SuperSet ELISA Kits are a range of specially designed ELISA Assays with the pharmaceutical and biotech research industries in mind. Focusing on the high quality monoclonal antibodies and purified standards, SuperSet ELISA Kits are key tools for immunology research. Each kit contains a highly optimised antibody pair as well as a purified recombinant protein for the detection of your analyte in cell culture supernatants. The SuperSet ELISA Kits can also be used for the detection of analytes in more complex matrices such as serum and plasma. Sensitivity: 31.25 pg/ml - 1000 pg/ml. Range: 12 pg/ml Protein function: Associates with EBI3 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T- helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1/ITGAL/ERK-dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up- regulation, an event crucial to TH1 cell commitment. It suppresses the expression of GATA3, the inhibitor TH1 cells development. In CD8 T-cells, it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL27 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of proinflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1. IL-27 has also an effect on cytokine production, it suppresses proinflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T- cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9. In vein endothelial cells, it induces IRF1/interferon regulatory factor 1 and increase the expression of MHC class II transactivator/CIITA with resulting up-regulation of major histocompatibility complex class II. IL-27 also demonstrates antiviral activity with inhibitory properties on HIV-1 replication. [The UniProt Consortium] Materials required but not provided: 96 well Microtiter plates: e.g. Nunc Maxisorp Cat # 468667 * Note: the use of ELISA plates which are not high affinity binding will result in lower performances. Reconstitution Buffer: 1xPBS, 0.09% Azide * Coating Buffer: 1xPBS, pH 7.2-7.4 * Wash Buffer: 1xPBS, 0.05% Tween20 * Blocking Buffer: 1xPBS, 5% BSA * Standard Dilution Buffer: 1xPBS, 1% BSA * Secondary Antibody Dilution Buffer: 1xPBS, 1% BSA * Note: Supplementation with 10% Animal Serum e.g. FCS for assaying serum, plasma or other body fluids samples may be necessary. HRP Dilution Buffer: 1xPBS, 1% BSA, 0.1% Tween20 * Stop Reagent: 1N Sulfuric Acid
Schlagworte: p28, IL27, IL27A, IL27-A, IL-27-A, Interleukin-30, IL-27 subunit alpha, Interleukin-27 subunit alpha
Hersteller: ELISA Genie
Hersteller-Nr: HUDC0129-1


Anwendung: ELISA
Reaktivität: Human
Format: Solid Phase

Handhabung & Sicherheit

Lagerung: +4°C
Versand: +4°C (International: +4°C)
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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