IL-34, BioAssay(TM) ELISA Kit (Human) (Interleukin-34)

IL-34, BioAssay(TM) ELISA Kit (Human) (Interleukin-34)
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
352931.96 96 tests - -

3 - 19 Werktage*

947,00 €
 
Interleukin-34, or IL-34 is a protein belonging to a group of cytokines called interleukins. It... mehr
Produktinformationen "IL-34, BioAssay(TM) ELISA Kit (Human) (Interleukin-34)"
Interleukin-34, or IL-34 is a protein belonging to a group of cytokines called interleukins. It is mapped to 16q22.1. The discovery of IL-34 protein in the red pulp of the spleen suggests involvement in growth and development of myeloid cells, consistent with its activity on monocytes. IL-34 is a recently discovered cytokine that acts on tissue resident macrophages and Langerhans cells upon binding the receptor for CSF-1, CSF-1R. It also can promote the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor. In addition, it promotes the development, survival, and function of microglia and Langerhans cells, therefore, this cytokine may predominately function in brain and skin biology. Sample Type: Cell culture supernates, serum and plasma (heparin, EDTA), Intended Use: For quantitative detection of human IL-34 in cell culture supernates, serum and plasma (heparin, EDTA). Sensitivity: <10pg/ml, Range: 62.5-4000pg/ml, Specificity: Natural and recombinant human IL-34, Test Principle: This human IL-34 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-34 has been precoated onto 96-well plates. Standards(E.coli, N21-P242) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-34 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-34 amount of sample captured in plate. Kit Components: 96-well plate precoated with human IL-34 antibody, 1, Lyophilized recombinant human IL-34 standard, 10ng/tube×2, Biotinylated human IL-34 antibody, 130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100), Sample diluent buffer, 30ml, Antibody diluent buffer, 12ml, ABC diluent buffer, 12ml, TMB color developing agent, 10ml, TMB stop solution, 10ml, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Schlagworte: IL34, IL-34, C16orf77, Interleukin-34
Hersteller: United States Biological
Hersteller-Nr: 352931

Eigenschaften

Anwendung: ELISA
Wirt: Human
Spezies-Reaktivität: human
Format: Solid Phase

Handhabung & Sicherheit

Lagerung: vT
Versand: +4°C (International: °C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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