Epiregulin BioAssay(TM) ELISA Kit (Porcine)

Epiregulin BioAssay(TM) ELISA Kit (Porcine)
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
396648.96 96 tests - -

3 - 19 Werktage*

704,00 €
Epiregulin (EPR) is a protein that in humans is encoded by the EREG gene. It is a member of the... mehr
Produktinformationen "Epiregulin BioAssay(TM) ELISA Kit (Porcine)"
Epiregulin (EPR) is a protein that in humans is encoded by the EREG gene. It is a member of the epidermal growth factor family. Epiregulin can function as a ligand of epidermal growth factor receptor (EGFR), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. The secondary structure at the C-terminal epiregulin is different from other epidermal growth factor family ligands because of the lack of hydrogen bonds. The structural difference at the C-terminal may provide an explanation for the reduced binding affinity of epiregulin to the ERBB receptors. Sample Type: Cell culture supernates, serum, plasma (heparin or EDTA). Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Porcine Epiregulin. Sensitivity: <10pg/ml, Range: 156pg/ml-10,000pg/ml, Specificity: Recognizes natural and recombinant Porcine Epiregulin. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Porcine Epiregulin BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A monoclonal antibody from mouse specific for Porcine Epiregulin has been precoated onto 96-well plates. Standards(E.coli, V60-L108) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Porcine Epiregulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce, a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Porcine Epiregulin amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Porcine EREG antibody, 1x plate, Lyophilized recombinant Porcine EREG standard, 2x10ng/tube, Biotinylated anti-Porcine EREG antibody, 1x130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100), Sample diluent buffer, 1x30ml, Antibody diluent buffer, 1x12ml, ABC diluent buffer, 1x12ml, TMB color developing agent, 1x10ml, TMB stop solution, 1x10ml, PBS washing buffer Powder for 1000ml, 1x buffer, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Schlagworte: EREG
Hersteller: United States Biological
Hersteller-Nr: 396648


Anwendung: ELISA
Wirt: Porcine
Reaktivität: Swine
Format: Solid Phase

Handhabung & Sicherheit

Lagerung: vT
Versand: +4°C (International: °C)
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
Hier folgen Informationen zur Produktreferenz. mehr
Hier kriegen Sie ein Zertifikat
oder , um Analysenzertifikate anzufordern.
Bewertungen lesen, schreiben und diskutieren... mehr
Kundenbewertungen für "Epiregulin BioAssay(TM) ELISA Kit (Porcine)"
Bewertung schreiben
oder , um eine Produktbewertung abzugeben.
Zuletzt angesehen