Epiregulin, BioAssay(TM) ELISA Kit (Mouse)

Epiregulin, BioAssay(TM) ELISA Kit (Mouse)
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
352843.96 96 tests - -

3 - 19 Werktage

704,00 €
 
Epiregulin (EPR) is a protein that in humans is encoded by the EREG gene. It is a member of the... mehr
Produktinformationen "Epiregulin, BioAssay(TM) ELISA Kit (Mouse)"
Epiregulin (EPR) is a protein that in humans is encoded by the EREG gene. It is a member of the epidermal growth factor family. Epiregulin can function as a ligand of epidermal growth factor receptor (EGFR), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. The secondary structure at the C-terminus epiregulin is different from other epidermal growth factor family ligands because of the lack of hydrogen bonds. The structural difference at the C-terminus may provide an explanation for the reduced binding affinity of epiregulin to the ERBB receptors. Sample Type: Cell culture supernates, serum and plasma (heparin, EDTA), Intended Use: For quantitative detection of mouse Epiregulin in cell culture supernates, serum and plasma (heparin, EDTA). Sensitivity: <10pg/ml, Range: 125-8000pg/ml, Specificity: Natural and recombinant mouse Epiregulin, Test Principle: This mouse Epiregulin BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Epiregulin has been precoated onto 96-well plates. Standards (E.coli, V53-L101) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Epiregulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Epiregulin amount of sample captured in plate. Kit Components: 96-well plate precoated with mouse Epiregulin antibody, 1, Lyophilized recombinant mouse Epiregulin standard, 10ng/tube×2, Biotinylated mouse Epiregulin antibody, 130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100), Sample diluent buffer, 30ml, Antibody diluent buffer, 12ml, ABC diluent buffer, 12ml, TMB color developing agent, 10ml, TMB stop solution, 10ml, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Schlagworte: Ereg
Hersteller: United States Biological
Hersteller-Nr: 352843

Eigenschaften

Anwendung: ELISA
Reaktivität: Mouse
Format: Solid Phase

Handhabung & Sicherheit

Lagerung: vT
Versand: +4°C (International: °C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
Hier kriegen Sie ein Zertifikat
oder , um Analysenzertifikate anzufordern.
Bewertungen lesen, schreiben und diskutieren... mehr
Kundenbewertungen für "Epiregulin, BioAssay(TM) ELISA Kit (Mouse)"
Bewertung schreiben
oder , um eine Produktbewertung abzugeben.
Hier folgen Informationen zur Produktreferenz. mehr
Zuletzt angesehen