Endothelin BioAssay(TM) ELISA Kit, Rat

Sandwich ELISA quantitative detection kit for Rat Endothelin in cell culture supernates, serum and plasma (heparin, EDTA). The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of human endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure (hypertension) and heart disease. , Assay Range:, 3.9pg/ml-250pg/ml, Sensitivity: < 0.5pg/ml, Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: P22388, Entrez Gene ID: 24323, Kit Components: 96-well plate pre-coated with anti-Endothelin, Rat, Lyophilized recombinant Endothelin, Rat standard, 10ng/tube, 2Ea, USB Cat No Kit Component, 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution), 143697 Sample diluent buffer, 30ml, 143698 Antibody diluent buffer, 12ml, 143699 ABC diluent buffer, 12ml, 143700 TMB color developing agent, 10ml, 143701 TMB stop solution, 10ml, Storage and Handling: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.