Endostatin (ES) BioAssay(TM) ELISA Kit (Mouse)

Endostatin (ES) BioAssay(TM) ELISA Kit (Mouse)
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
024795.96 96 tests - -

1 - 19 Werktage

806,00 €
 
Endostatin (ES) BioAssay(TM) ELISA Kit (Mouse) is a sandwich enzyme immunoassay for in vitro... mehr
Produktinformationen "Endostatin (ES) BioAssay(TM) ELISA Kit (Mouse)"
Endostatin (ES) BioAssay(TM) ELISA Kit (Mouse) is a sandwich enzyme immunoassay for in vitro quantitative measurement of ES in mouse serum, plasma and other biological fluids. Detection Range: 0.156-10ng/ml, Sensitivity: 0.056ng/ml, Intra-Assay CV: < 10% , Inter-Assay CV: < 12%, Kit Components: *024795A: Microtiter Strip, 1x96 wells, Pre-coated, ready to use, *024795B: Standard, 2x1 vial, 024795C: Standard Diluent, 1x20ml, *024795D: Detection Reagent A, 1x120ul, *024795E: Detection Reagent B, 1x120ul, 024795F: Assay Diluent A, 1x12ml, 024795G: Assay Diluent B, 1x12ml, 024795H: TMB Substrate, 1x9ml, *024795J: Positive Control, 1x1vial, 024795K: Stop Solution, 1x6ml, 024795L: Wash Buffer, 30X, 1x20ml, Precaution: The Stop Solution (024795K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *024795A, *024795B, *024795D, *024795E and *024795J at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards,, 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C., 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C., 4. Aspirate and wash 3 times., 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C., 6. Aspirate and wash 5 times., 7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C., 8. Add 50ul Stop Solution. Read at 450nm immediately.
Schlagworte: Col18a1
Hersteller: United States Biological
Hersteller-Nr: 024795

Eigenschaften

Anwendung: ELISA
Reaktivität: Mouse
Format: Solid Phase

Handhabung & Sicherheit

Lagerung: vT
Versand: +4°C (International: +4°C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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