Endostatin, BioAssay(TM) ELISA Kit (Mouse)

Endostatin, BioAssay(TM) ELISA Kit (Mouse)
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
352835.96 96 tests - -

3 - 19 Werktage*

947,00 €
 
Endostatin is a naturally-occurring 20kD C-terminal fragment derived from type XVIII collagen. It... mehr
Produktinformationen "Endostatin, BioAssay(TM) ELISA Kit (Mouse)"
Endostatin is a naturally-occurring 20kD C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And It is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo. Sample Type: Cell culture supernates, serum and plasma (heparin, EDTA), Intended Use: For quantitative detection of mouse Endostatin in cell culture supernates, serum and plasma (heparin, EDTA). Sensitivity: <10pg/ml, Range: 156-10,000pg/ml, Specificity: Natural and recombinant mouse Endostatin, Test Principle: This mouse Endostatin BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Endostatin has been precoated onto 96-well plates. Standards(NSO, H1591-K1774) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Endostatin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Endostatin amount of sample captured in plate. Kit Components: 96-well plate precoated with mouse Endostatin antibody, 1, Lyophilized mouse Endostatin standard, 10ng/tube×2, Biotinylated mouse Endostatin antibody, 130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100), Sample diluent buffer, 30ml, Antibody diluent buffer, 12ml, ABC diluent buffer, 12ml, TMB color developing agent, 10ml, TMB stop solution, 10ml, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Hersteller: United States Biological
Hersteller-Nr: 352835

Eigenschaften

Anwendung: ELISA
Spezies-Reaktivität: mouse
Format: Solid Phase

Handhabung & Sicherheit

Lagerung: vT
Versand: +4°C (International: °C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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