CEA, BioAssay(TM) ELISA Kit (Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, Carcinoembry

CEA, BioAssay(TM) ELISA Kit (Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, Carcinoembry
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
C1300-06A.96 96 tests - -

1 - 19 Werktage

910,00 €
 
Carcinoembryonic antigen (CEA) was first described in 1965 by Gold and Freedman when they... mehr
Produktinformationen "CEA, BioAssay(TM) ELISA Kit (Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, Carcinoembry"
Carcinoembryonic antigen (CEA) was first described in 1965 by Gold and Freedman when they identified an antigen that was present in both fetal colon and colon adenocarcinoma but that appeared to be absent from healthy adult colon. CEA is a glycoprotein containing approximately 50% carbohydrate with a molecular weight of 180kD. CEA and related genes make up the CEA family belonging to the immunoglobulin superfamily. In humans, the carcinoembryonic antigen family consists of 29 genes, 18 of which are normally expressed. CEA has proven to be a suitable target antigen for the detection of primary and metastatic colorectal and some other carcinomas. Although sera from healthy individuals and from patients with other diseases generally had low levels of CEA, CEA is one of the most widely used tumor markers worldwide. Its main application is mostly in gastrointestinal cancers, especially in colorectal malignancy. CEA assays are now generally accepted clinically as a useful and cost- efficient tool in monitoring of colon cancer following surgery. , Intended Use:, Human Carcinoembryonic Antigen (human CEA) ELISA kit is to be used for the in vitro quantitative determination of human CEA in human serum, human plasma, cell lysate and buffered solution. The assay will recognize native CEA. This kit has been configured for research use only and is not to be used in diagnostic procedures. Sensitivity: The minimal detectable dose of human CEA was calculated to be 0.15ng/ml, by subtracting two standard deviations from the mean of 10 zero standard replicates (ELISA buffer, S0) and intersecting this value with the standard curve obtained in the same calculation. Specificity: The following substances were tested and found to have no cross-reactivity: human, Test Principle: The design of this assay is based on a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to human CEA. Samples are pippetted into these wells. Nonbound CEA and other components of the sample should be removed by washing, then biotin-conjugated monoclonal antibody specific to CEA added. In order to quantitatively determine the amount of CEA present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) should be added to each microplate well. The final step, a TMB-substrate solution added to each well. Finally, a sulfuric acid solution is added and the resulting yellow colored product is measured at 450nm. Since the increases in absorbency is directly proportional to the amount of captured CEA. Kit Components: Microplate: 1x96 wells, Incubation buffer: 1x30ml, Washing buffer, (10x): 1x100ml, Standard protein: 1 glass vial lyophilized, Standard/sample dilution buffer: 1x25ml, Second antibody: 1 glass vial lyophilized, AV-HRP: 1x150ul, Secondary antibody/AV-HRP dilution buffer: 1x25ml, Substrate (TMB): 1x20ml, Stop solution: 1x20ml, Storage and Stability: Store components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Hersteller: United States Biological
Hersteller-Nr: C1300-06A

Eigenschaften

Anwendung: ELISA
Format: Solid Phase

Handhabung & Sicherheit

Lagerung: +4°C
Versand: +4°C (International: +4°C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
Hier kriegen Sie ein Zertifikat
oder , um Analysenzertifikate anzufordern.
Bewertungen lesen, schreiben und diskutieren... mehr
Kundenbewertungen für "CEA, BioAssay(TM) ELISA Kit (Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, Carcinoembry"
Bewertung schreiben
oder , um eine Produktbewertung abzugeben.
Hier folgen Informationen zur Produktreferenz. mehr
Zuletzt angesehen