CD47 (Cluster of Differentiation 47), BioAssay(TM) ELISA Kit (Human)

CD47 (Cluster of Differentiation 47), BioAssay(TM) ELISA Kit (Human)
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
352768.96 96 tests - -

3 - 19 Werktage*

1.212,00 €
 
CD47 (Cluster of Differentiation 47), also known as integrin associated protein (IAP), is a... mehr
Produktinformationen "CD47 (Cluster of Differentiation 47), BioAssay(TM) ELISA Kit (Human)"
CD47 (Cluster of Differentiation 47), also known as integrin associated protein (IAP), is a transmembrane protein that in humans is encoded by the CD47 gene. It belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPalpha). CD47 is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. Furthermore, it plays a key role in immune and angiogenic responses. Also CD47 is ubiquitously expressed in human cells and has found to be overexpressed in many different tumor cells. Sample Type: Cell culture supernates and serum. Intended Use: For quantitative detection of human CD47 in cell culture supernates and serum. Sensitivity: <10pg/ml, Range: 125-8000pg/ml, Specificity: Natural and recombinant human CD47, Test Principle: This human CD47 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD47 has been precoated onto 96-well plates. Standards (NSO, Q19 - P139) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD47 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD47 amount of sample captured in plate. Kit Components: 96-well plate precoated with human CD47 antibody, 1, Lyophilized recombinant human CD47 standard, 10ng/tube×2, Biotinylated human CD47 antibody, 130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100), Sample diluent buffer, 30ml, Antibody diluent buffer, 12ml, ABC diluent buffer, 12ml, TMB color developing agent, 10ml, TMB stop solution, 10ml, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Schlagworte: IAP, MER6, CD47, Protein MER6, Integrin-associated protein, Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3
Hersteller: United States Biological
Hersteller-Nr: 352768

Eigenschaften

Anwendung: ELISA
Wirt: Human
Spezies-Reaktivität: human
Format: Solid Phase

Handhabung & Sicherheit

Lagerung: vT
Versand: +4°C (International: °C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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