CD25/IL-2sR alpha BioAssay(TM) ELISA Kit, Mouse

Sandwich ELISA quantitative detection kit for Mouse CD25/IL-2sR alpha in cell culture supernates, serum and plasma (heparin, EDTA). CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. CD25 has been used as a marker to identify CD4+FoxP3+ regulatory T cells in mice. By in situ hybridization, the IL2R gene was mapped to 10p15-p14. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression. , Assay Range:, 78pg/ml-5000pg/ml, Sensitivity: < 5 pg/ml, Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: P01590, Entrez Gene ID: 16184, Kit Components: 96-well plate pre-coated with anti-CD25/IL-2sR alpha, Mouse, Lyophilized recombinant CD25/IL-2sR alpha, Mouse standard, 10ng/tube, 2Ea, USB Cat No Kit Component, 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution), 143697 Sample diluent buffer, 30ml, 143698 Antibody diluent buffer, 12ml, 143699 ABC diluent buffer, 12ml, 143700 TMB color developing agent, 10ml, 143701 TMB stop solution, 10ml, Storage and Handling: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.