CD21/CR2, BioAssay(TM) ELISA Kit (Human) (Complement Receptor Type 2)

CD21/CR2, BioAssay(TM) ELISA Kit (Human) (Complement Receptor Type 2)
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
352757.96 96 tests - -

3 - 19 Werktage

704,00 €
 
Complement receptor type 2 (CR2), also known as C3DR or CD21, is a protein that in humans is... mehr
Produktinformationen "CD21/CR2, BioAssay(TM) ELISA Kit (Human) (Complement Receptor Type 2)"
Complement receptor type 2 (CR2), also known as C3DR or CD21, is a protein that in humans is encoded by the CR2 gene. It is mapped to 1q32.2. CR2 is involved in the complement system. It binds to iC3b(inactive derivative of C3b), C3dg, or C3d. CR2 is the membrane protein on B lymphocytes to which the Epstein-Barr virus (EBV) binds during infection of these cells. CR2, together with CD19, CD81, and CD225, forms the B-cell coreceptor complex, which lowers the activation threshold of the B-cell antigen receptor. Genetic variations in this gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9). Sample Type: Cell culture supernates, serum and plasma (heparin, EDTA), Intended Use: For quantitative detection of human CD21 in cell culture supernates, serum and plasma (heparin, EDTA). Sensitivity: <20pg/ml, Range: 0.78-50ng/ml, Specificity: Natural and recombinant human CD21, Test Principle: This human CD21 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD21 has been precoated onto 96-well plates. Standards(CHO, Ile21-71) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD21 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD21 amount of sample captured in plate. Kit Components: 96-well plate precoated with human CD21 antibody, 1, Lyophilized recombinant human CD21 standard, 50ng/tube×2, Biotinylated human CD21 antibody, 130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100), Sample diluent buffer, 30ml, Antibody diluent buffer, 12ml, ABC diluent buffer, 12ml, TMB color developing agent, 10ml, TMB stop solution, 10ml, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Schlagworte: CR2, Cr2, C3DR, CD21, EBV receptor, Complement C3d receptor, Complement receptor type 2, Epstein-Barr virus receptor
Hersteller: United States Biological
Hersteller-Nr: 352757

Eigenschaften

Anwendung: ELISA
Wirt: Human
Reaktivität: Human
Format: Solid Phase

Handhabung & Sicherheit

Lagerung: vT
Versand: +4°C (International: °C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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