Carcinoembryonic Antigen, Human (CEA, Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, CEA

Carcinoembryonic Antigen, Human (CEA, Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, CEA
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
C1300-05H.96 96 tests - -

1 - 19 Werktage

683,00 €
 
Carcinoembroyonic antigen (CEA) is a cell-surface 200kD glycoprotein. In 1969, it was reported... mehr
Produktinformationen "Carcinoembryonic Antigen, Human (CEA, Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, CEA"
Carcinoembroyonic antigen (CEA) is a cell-surface 200kD glycoprotein. In 1969, it was reported that plasma CEA was elevated in 35 of 36 patients with adenocarcinoma of the colon and that CEA titers decreased after successful surgery. Normal levels were observed in all patients with other forms of cancer or benign diseases. Subsequent studies have not confirmed these initial findings, and it is now understood that elevated levels of CEA are found in many cancers. Increased levels of CEA are observed in more than 30% of patients with cancer of the lung, liver, pancreas, breast, colon, head or neck, bladder, cervix, and prostate. Elevated plasma levels are related to the stage and extent of the disease, the degree of differentiation of the tumor, and the site of metastasis. CEA is also found in normal tissue. Sample Type: Serum, Intended Use: For the quantitative research of the Cancer Antigen CEA concentration in human serum. Test Principle: The CEA ELISA test is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay system utilizes a monoclonal antibody directed against a distinct antigenic determinant on the intact CEA molecule is used for solid phase immobilization (on the microtiter wells). A goat anti-CEA antibody conjugated to horseradish peroxidase (HRP) is in the antibody-enzyme conjugate solution. The test sample is allowed to react simultaneously with the two antibodies, resulting in the CEA molecules being sandwiched between the solid phase and enzyme-linked antibodies. After a 1 hour incubation at room temperature, the wells are washed with water to remove unbound labeled antibodies. A solution of TMB Reagent is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of Stop Solution changing the color to yellow. The concentration of CEA is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm. Kit Components: Antibody-coated microtiter plate 1x96 wells. Standards 0ng/ml, 3ng/ml, 12ng/ml, 30ng/ml, 60ng/ml, 120ng/ml CEA. , 1ml each, ready to use Enzyme Conjugate Reagent , TMB Reagent (One-Step) 1x13ml , Stop Solution (1N HCl), 1x11 ml, Storage and Stability: Store all components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Schlagworte: CEA, CD66e, CEACAM5, Meconium antigen 100, Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5
Hersteller: United States Biological
Hersteller-Nr: C1300-05H

Eigenschaften

Anwendung: ELISA
Reaktivität: Human
Format: Solid Phase

Handhabung & Sicherheit

Lagerung: +4°C
Versand: +4°C (International: +4°C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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