Cadherin-2/N-Cadherin, BioAssay(TM) ELISA Kit (Mouse)

Cadherin-2/N-Cadherin, BioAssay(TM) ELISA Kit (Mouse)
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
352724.96 96 tests - -

3 - 19 Werktage*

704,00 €
 
Cadherin-2(CDH2), also known as neural cadherin(NCAD), is a protein that in humans is encoded by... mehr
Produktinformationen "Cadherin-2/N-Cadherin, BioAssay(TM) ELISA Kit (Mouse)"
Cadherin-2(CDH2), also known as neural cadherin(NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. Sample Type: Cell culture supernates, serum and plasma (heparin, EDTA), Intended Use: For quantitative detection of mouse Cadherin-2 in cell culture supernates, serum and plasma (heparin, EDTA). Sensitivity: <20pg/ml, Range: 0.78-50ng/ml, Specificity: Natural and recombinant mouse Cadherin-2, Test Principle: This mouse Cadherin-2 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Cadherin-2 has been precoated onto 96-well plates. Standards(NSO) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cadherin-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Cadherin-2 amount of sample captured in plate. Kit Components: 96-well plate precoated with mouse Cadherin-2 antibody, 1, Lyophilized recombinant mouse Cadherin-2 standard, 50ng/tube×2, Biotinylated mouse Cadherin-2 antibody, 130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100), Sample diluent buffer, 30ml, Antibody diluent buffer, 12ml, ABC diluent buffer, 12ml, TMB color developing agent, 10ml, TMB stop solution, 10ml, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Schlagworte: Cdh2, CD325, N-cadherin, Cadherin-2, Neural cadherin
Hersteller: United States Biological
Hersteller-Nr: 352724

Eigenschaften

Anwendung: ELISA
Reaktivität: Mouse
Format: Solid Phase

Handhabung & Sicherheit

Lagerung: vT
Versand: +4°C (International: °C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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