C-reactive Protein (CRP), BioAssay(TM) ELISA Kit (Rat)

C-reactive Protein (CRP), BioAssay(TM) ELISA Kit (Rat)
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
350977.96 96 tests - -

1 - 19 Werktage

714,00 €
C-reactive protein (CRP) is an annular, pentameric protein found in the blood plasma, the levels... mehr
Produktinformationen "C-reactive Protein (CRP), BioAssay(TM) ELISA Kit (Rat)"
C-reactive protein (CRP) is an annular, pentameric protein found in the blood plasma, the levels of which rise in response to inflammation. Its physiological role is to bind to lysophosphatidyl- choline expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system via the C1Q complex. CRP is synthesized by the liver in response to factors released by macrophages and fat cells. It is not related to C-peptide or protein C. The rat CRP gene encodes a 230 amino acid (aa) precursor with a signal peptide of 19aa and the mature polypeptide of 211aa. Rat CRP shares 64% and 70% aa sequence homology with human and mouse CRP respectively. Rat CRP is a glycoprotein and contains a covalently linked dimer in the pentameter. Sample Type: Serum and plasma, Intended Use: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of CRP in rat serum, plasma, and other biological fluids not being tested. Sensitivity: 29pg/ml, Range: 163.8-40,000pg/ml, Specificity: This assay has high sensitivity and excellent specificity for detection of rat CRP. Precision: Intra CV<10%, Inter CV <15%, Test Principle: This assay employs the fast quantitative sandwich enzyme immunoassay technique. Antibody specific for Rat CRP has been pre-coated onto a microplate. Standards, samples and rat CRP mix are pipetted into the wells and any CRP present is bound by the immobilized antibody. Following a wash to remove any unbound reagent, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CRP bound in the initial step. The color development is stopped and the intensity of the color is measured. Kit Components: Assay plate (12x8 coated Microwells)-1, Standard-2x10uL, Rat CRP Mix-4x8uL, Assay diluent (5x concentrate)-1x15mL, Wash buffer (20x concentrate)-1x25mL, TMB substrate-1x12mL, Stop solution-1x8mL, Adhensive strip-2, Instruction manual-1. Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Schlagworte: Crp, Ptx1, C-reactive protein
Hersteller: United States Biological
Hersteller-Nr: 350977


Anwendung: ELISA
Reaktivität: Rat
Format: Solid Phase

Handhabung & Sicherheit

Lagerung: vT
Versand: +20°C (International: °C)
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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