AGP1/Alpha 1 Acid Glycoprotein BioAssay(TM) ELISA Kit (Human)

Alpha-1-acid glycoprotein, or orosomucoid, is a relatively abundant plasma protein synthesized in the liver. It has a molecular weith of 40,000 Da and is a single polypeptide chain of about 180aa. AGP is considered an acute phase reactant, its plasma levels increase several fold during acute infections. It belongs to the lipocalin family and binds numerous basic and neutral lipophilic drugs and steroid hormones. And this gene is located in 9q34.3-qter. Sample Type: Cell lysates, cell culture supernates, serum, plasma (Heparin or EDTA). Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Human AGP1/alpha 1 acid glycoprotein. Sensitivity: <150pg/ml, Specificity: Recognizes natural and recombinant Human AGP1/alpha 1 acid glycoprotein. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Human AGP1/alpha 1 acid glycoprotein ELISA Kit was based on standard sandwich enzyme- linked immune-sorbent assay technology. A specific for AGP1/alpha 1 acid glycoprotein has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: Q19-S201) and test samples are added to the wells, a biotinylated detection specific for AGP1/alpha 1 acid glycoprotein is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human AGP1/alpha 1 acid glycoprotein amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Human ORM1 antibody, 1x plate, Lyophilized recombinant Human ORM1 standard, 2x100ng/tube, Biotinylated anti-Human ORM1 antibody, 1x130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100), Sample diluent buffer, 1x30ml, Antibody diluent buffer, 1x12ml, ABC diluent buffer, 1x12ml, TMB color developing agent, 1x10ml, TMB stop solution, 1x10ml, PBS washing buffer Powder for 1000ml, 1x buffer, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.