ELISA und Immunassays

Enzyme-linked Immunosorbent Assay (ELISA) ist ein biochemisches Verfahren, um das Vorhandensein eines Antikörpers oder eines Antigens in einer Probe nachzuweisen. Ein indirekter ELISA wird verwendet, um die Menge von Antikörpern gegen ein spezifisches Antigen in einer Probe zu bestimmen. Ein Sandwich-ELISA dient hingegen dazu, die Konzentration des Analyten unter Verwendung von Antikörpern, die an den Analyten (und einer Standard) binden, zu bestimmen. Eine andere Möglichkeit zur Bestimmung der Analyt-Konzentration ist ein kompetitiver ELISA, oft als EIA bezeichnet. Diese Technik wird bei kleinen Analyten bevorzugt.

Enzyme-linked Immunosorbent Assay (ELISA) ist ein biochemisches Verfahren, um das Vorhandensein eines Antikörpers oder eines Antigens in einer Probe nachzuweisen. Ein indirekter ELISA wird... mehr erfahren »
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ELISA und Immunassays

Enzyme-linked Immunosorbent Assay (ELISA) ist ein biochemisches Verfahren, um das Vorhandensein eines Antikörpers oder eines Antigens in einer Probe nachzuweisen. Ein indirekter ELISA wird verwendet, um die Menge von Antikörpern gegen ein spezifisches Antigen in einer Probe zu bestimmen. Ein Sandwich-ELISA dient hingegen dazu, die Konzentration des Analyten unter Verwendung von Antikörpern, die an den Analyten (und einer Standard) binden, zu bestimmen. Eine andere Möglichkeit zur Bestimmung der Analyt-Konzentration ist ein kompetitiver ELISA, oft als EIA bezeichnet. Diese Technik wird bei kleinen Analyten bevorzugt.

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Interleukin-6 (human) ELISA Kit
Interleukin-6 (human) ELISA Kit

Artikelnummer: Cay501030-96

Interleukin-6 (IL-6) is produced by multiple cell types, including monocytes, macrophages, and T cells and is involved in many defense mechanisms including the immune response, hematopoiesis, bone metabolism, and acute phase reactions. IL-6 promotes the proliferation of activated B-cells, acts as a B-cell...
Schlagworte: CDF, IL6, IL-6, BSF-2, IFNB2, IFN-beta-2, Interleukin-6, Interferon beta-2, Hybridoma growth factor, CTL differentiation...
Anwendung: ELISA
357,00 €
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Cyclic AMP Select ELISA Kit
Cyclic AMP Select ELISA Kit

Artikelnummer: Cay501040-96

Cayman's cAMP Select assay is a competitive ELISA that permits cAMP measurements within the standard curve range of 0.09-200 pmol/ml and a sensitivity (80% B/B0) of approximately 0.6 pmol/ml. Acetylation of samples is not required in this highly sensitive assay where culture media, urine, tissue, and plasma samples...
Schlagworte: Adenosine 3',5'-cyclic mononucleotide, Adenosine 3',5'-cyclic monophosphate, cAMP EIA Kit
Anwendung: ELISA
Reaktivität: Broad
ab 374,00 €
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Leukotriene E4 ELISA Kit
Leukotriene E4 ELISA Kit

Artikelnummer: Cay501060-96

Leukotriene E4 (LTE4) is a product of the 5-lipoxygenase (5-LO) pathway in activated mast cells, eosinophils, and monocytes. LTA4, the primary 5-LO metabolite, is rapidly converted to LTC4 and sequentially to LTD4 and LTE4. While plasma levels of LTC4 are virtually undetectable, exogenously administered LTC4 is...
Schlagworte: LTE4 EIA Kit
Anwendung: ELISA
Reaktivität: Human
ab 400,00 €
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Aldosterone ELISA Kit
Aldosterone ELISA Kit

Artikelnummer: Cay501090-96

Aldosterone, a steroid hormone secreted by the adrenal cortex, is the principle mineralocorticoid controlling sodium and potassium balance. Serum aldosterone levels in normal upright adult individuals are generally less than 300 pg/ml. Only a fraction of urinary aldosterone is excreted intact, thus urinary excretion...
Schlagworte: Aldosterone ELISA
Anwendung: ELISA
ab 344,00 €
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Maresin 1 ELISA Kit
Maresin 1 ELISA Kit

Artikelnummer: Cay501150-96

Maresins (macrophage mediators in resolving inflammation) are members of a class of potent anti-inflammatory and pro-resolving mediators that are synthesized by macrophages in the presence of docosahexaenoic acid (DHA). Maresin 1 (MaR1) is produced via oxidation of endogenous DHA by 12-lipoxygenase. MaR1 has been...
Schlagworte: 7(R)-MaR1, 7(R)-Maresin 1 EIA, MaR1
Anwendung: ELISA
Reaktivität: Broad
494,00 €
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Corticosterone ELISA Kit
Corticosterone ELISA Kit

Artikelnummer: Cay501320-480S

Corticosterone is a glucocorticoid produced by the adrenal cortex in response to ACTH (corticotropic hormone) and is the precursor to aldosterone. The production of glucorcorticoids is increased by stress, therefore, corticosterone can be used as a biomarker of stress. Biologists often measure corticosterone in...
Schlagworte: 11beta,21-DHP, 11beta,21-dihydroxy Progesterone, 17-deoxy Cortisol, Corticosterone EIA Kit
Anwendung: ELISA
ab 307,00 €
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Myeloperoxidase (human) ELISA Kit
Myeloperoxidase (human) ELISA Kit

Artikelnummer: Cay501410-96

Myeloperoxidase (MPO) is a peroxidase enzyme with roles in innate immunoresponse and various diseases. Cayman's MPO (human) ELISA Kit is a sandwich assay that can be used to measure MPO in plasma and serum without prior sample purification. This assay has been validated using plasma and serum from healthy volunteers...
Schlagworte: MPO (human) EIA Kit
Anwendung: ELISA
469,00 €
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11-Oxoetiocholanolone ELISA Kit
11-Oxoetiocholanolone ELISA Kit

Artikelnummer: Cay501420-96

11-Oxoetiocholanolone is a metabolite of cortisol that is found in the feces of ungulates and other animals. Stress-induced glucocorticoids (cortisol, corticosterone) traditionally have been measured through blood sampling, but the collection process is known to cause stress in animals, interfering with experimental...
Schlagworte: 11-Oxoetiocholanolone EIA Kit
Anwendung: ELISA
469,00 €
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Citrullinated Histone H3 (Clone 11D3) ELISA Kit
Citrullinated Histone H3 (Clone 11D3) ELISA Kit

Artikelnummer: Cay501620-96

Histone H3 is citrullinated by peptidylarginine deiminase 4 (PAD4) at residues R2, R8, and R17 during the processes of epigenetic chromatin remodeling and neutrophil extracellular trap (NET) formation. In vitro, citrullinated H3 (CitH3) is released from neutrophils that have been stimulated with calcium ionophore...
Schlagworte: CitH3 (Clone 11D3) EIA Kit
Anwendung: ELISA
619,00 €
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Prostaglandin D2-MOX ELISA Kit
Prostaglandin D2-MOX ELISA Kit

Artikelnummer: Cay512011-96

Prostaglandin D2 (PGD2) is biosynthesized in the brain by a soluble, 26 kD glutathione-independent lipocalin-type PGD2 synthase. PGD2 accumulates in the cerebrospinal fluid (CSF), where it induces physiologic sleep in rats and humans. PGD2 is also synthesized by mast cells and leukocytes by a cellular, myeloid-type,...
Schlagworte: PGD2-MOX EIA Kit
Anwendung: ELISA
ab 338,00 €
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Prostaglandin E2 ELISA Kit - Monoclonal
Prostaglandin E2 ELISA Kit - Monoclonal

Artikelnummer: Cay514010-480

Prostaglandin E2 (PGE2) is a primary product of arachidonic acid metabolism in many cells. Like most eicosanoids, it does not exist preformed in any cellular reservoir. When cells are activated or exogenous free arachidonate is supplied, PGE2 is synthesized de novo and released into the extracellular space. In vivo,...
Schlagworte: PGE2 EIA Kit - Monoclonal
Anwendung: ELISA
ab 344,00 €
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Prostaglandin Screening ELISA Kit
Prostaglandin Screening ELISA Kit

Artikelnummer: Cay514012-96

This assay was developed for screening applications in which the relative amount of prostaglandin (PG) production for a large number of cell culture samples must be determined. Our PGE2 assay (Cay-514010) is unparalleled in sensitivity and specificity, but the steep standard curve can make screening many samples...
Schlagworte: PG Screening EIA Kit
Anwendung: ELISA
ab 344,00 €
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