PDE10A, BioAssay(TM) Kit (cAMP and cAMP-inhibited cGMP 3',5'-cyclic Phosphodiesterase 10A)

Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE10A is a dual substrate PDE highly expressed in striatal medium spiny neurons. PDE10A inhibitors can improve the cognitive symptoms of schizophrenia, and exhibit potential therapeutic value for Huntington's Disease. The PDE10A Assay Kit is designed for identification of PDE10A inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE10A to the binding agent. Intended Use: Designed for identification of PDE10A inhibitors using fluorescence polarization. Test Principle: The PDE10A inhibitor screening assay kit comes in a convenient 96-well format, including purified PDE10A enzyme, fluorescently labeled PDE10A substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the PDE10A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE10A reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE10A for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization. Kit Components: PDE10A2: 1x700ng, FAM-Cyclic-3', 5'-AMP: 20uM: 1x50ul, PDE assay buffer: 1x12ml, Binding Agent: 1x100ul, Binding Agent Diluent (cAMP): 1x10ml, Black, low binding NUNC black microtiter plate: 1 plate, Storage and Stability: Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.