PARPtrap(TM) Combo Assay Kit for PARP1 and PARP2

PARPtrap(TM) Combo Assay Kit for PARP1 and PARP2
PARPtrap(TM) Combo Assay Kit for PARP1 and PARP2
   
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Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
BPS-78317 384 rxns - -

3 - 15 Werktage*

3.386,00 €
 
The PARPtrap(TM) Combo Assay Kit for PARP1 and PARP2 is a kit designed to measure DNA complex... mehr
Produktinformationen "PARPtrap(TM) Combo Assay Kit for PARP1 and PARP2"
The PARPtrap(TM) Combo Assay Kit for PARP1 and PARP2 is a kit designed to measure DNA complex formations of PARP1 and PARP2 enzymes in a high throughput screening assay using fluorescence polarization (FP). The key to the PARPtrap(TM) Combo Assay Kit are the fluorescent-labeled DNA probes recognized by PARP1 and PARP2. In the absence of ribosylation, PARP1/2 binds to the DNA fluorescent probe, forming a large complex and resulting in the emission of highly polarized light. However, after autoribosylation, PARP1/2 dissociates from the oligonucleotide duplex, which then rotates freely, emitting less polarized light (Fig. 1). Addition of a PARP inhibitor results in trapping of PARP1/2 to the fluorescent-labeled DNA, and increases the FP signal in a dose dependent manner. The combo kit is particularly useful to directly and specifically compare, in a single assay, the effect and potency of a compound on PARP1 and PARP2. The PARPtrap(TM) Combo Assay Kit for PARP1 and PARP2 is a homogeneous fluorescence polarization assay. The FP signal is measured using a fluorescent microplate reader capable of measuring fluorescence polarization.
Schlagworte: ARTD1, PARP-1, ADPRT 1, Poly[ADP-ribose] synthase 1, Poly [ADP-ribose] polymerase 1, NAD(+) ADP-ribosyltransferase 1, DNA ADP-ribosyltransferase PARP1, Protein poly-ADP-ribosyltransferase PARP1, ADP-ribosyltransferase diphtheria toxin-like 1,
Hersteller: BPS Bioscience
Hersteller-Nr: 78317

Eigenschaften

Anwendung: Small molecule screening, Drug discovery, HTS, IC50 determination
Reaktivität: Human
Format: Homog.

Handhabung & Sicherheit

Lagerung: -80°C
Versand: -80°C (International: °C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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