Mdm2 E3 Ligase TR-FRET Kit

Mdm2 E3 Ligase TR-FRET Kit
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
SBB-KF0030-KI01 1 kit -

3 - 9 Werktage*

1.433,00 €
 
South Bay Bio's MDM2 TR-FRET Ubiquitin Kit provides a fast and sensitive method monitoring... mehr
Produktinformationen "Mdm2 E3 Ligase TR-FRET Kit"
South Bay Bio's MDM2 TR-FRET Ubiquitin Kit provides a fast and sensitive method monitoring ubiquitin conjugation onto GST-MDM2 in solution, resulting from an enzymatic ubiquitin cascade without the need of running and staining an SDS gel. The kit enables continuous TR-FRET detection of ubiquitin chain formation onto MDM2 in a real-time detection setup, or in an end-point configuration if desired. TR-FRET uses the extended fluorescence emission decay lifetimes typical of rare-earth lanthanides to impart a short time-delay between FRET donor excitation and emission. This delay provides a means to separate "true" signal from short-lived background fluorescence, and reduce interference from compound fluorescence and other assay artifacts. Protein function: E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation (PubMed:12821780, PubMed:15053880, PubMed:15195100, PubMed:15632057, PubMed:16337594, PubMed:17290220, PubMed:19098711, PubMed:19219073, PubMed:19837670, PubMed:19965871, PubMed:20173098, PubMed:20385133, PubMed:20858735, PubMed:22128911). Ubiquitinates DCX, leading to DCX degradation and reduction of the dendritic spine density of olfactory bulb granule cells. [The UniProt Consortium]
Schlagworte: MDM2, Hdm2, EC=2.3.2.27, Oncoprotein Mdm2, Double minute 2 protein, p53-binding protein Mdm2, E3 ubiquitin-protein ligase Mdm2, RING-type E3 ubiquitin transferase Mdm2
Hersteller: South Bay Bio
Hersteller-Nr: KF0030

Eigenschaften

Anwendung: Enzyme assays
Spezies-Reaktivität: human
Format: Homog.

Handhabung & Sicherheit

Lagerung: -20°C
Versand: -20°C (International: -20°C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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