Cell Navigator(TM) Fluorimetric Lipid Droplet Assay Kit *Green Fluorescence*

Ex: 485 nm. Em: 520 nm. Lipid droplets, also referred to as lipid bodies, oil bodies or adiposomes, are lipid-rich cellular organelles that regulate the storage and hydrolysis of neutral lipids. They also serve as a reservoir of lipid source for many important biological processes such as fatty acid and cellular cholesterol for energy and membrane formation and maintenance. Abnormal accumulation of the cytoplasmic lipid droplets occurs in a variety of pathological conditions and can be an indicator of metabolic deficiency or pathogenesis. AAT Bioquest's Cell NavigatorT Fluorimetric Lipid Droplets Assay Kit is a simple assay that could quantitatively measure lipid droplet accumulation. Nile GreenT is used in the kit for lipophilic stain. Nile GreenT is intensely fluorescent in a lipid-rich environment while it has minimal fluorescence in aqueous media. It is an excellent vital stain for the detection of intracellular lipid droplets with fluorescence microscopy, flow cytometry or fluorescence microplate reader. Unlike Nile Red which has broad range of fluorescence spectrum, Nile GreenT stains intracellular lipid droplets with green fluorescence only. It can be used with other fluorescence dyes for multicolor staining. The green fluorescence signal could be read at Ex/Em=485/520 nm or observed using the filter set of FITC.