Cell Meter(TM) Fluorimetric Intracellular Peroxynitrite Assay Kit *Green Fluorescence*

Ex: 502 nm. Em: 535 nm. Soluble in: DMSO. Peroxynitrite (ONOO-) is a strong oxidizing species and a highly active nitrating agent. Peroxynitrite is formed from the reaction between superoxide radicals and nitric oxide generated in cells. It can cause damages to a wide array of biomolecules including proteins, enzymes, lipids and nucleic acids, eventually contributing to cell death. Meanwhile, peroxynitrite can also have protective activities in vivo by contributing to host-defense responses against invading pathogens. Therefore, peroxynitrite is an essential biological oxidant involved in a board range of physiological and pathological processes. Due to its extremely short half-life and low steady-state concentration, it has been challenging to detect and understand the role of peroxynitrite in biological systems. AAT Bioquest's DAX-J2T PON Green has been developed to address this umet need. It provides a sensitive tool to monitor ONOO- level in living cells. AAT Bioquest's DAX-J2T PON Green specifically reacts with intercellular ONOO- to generate a bright green fluorescent product. It can be used in fluorescence imaging, flow cytometry and fluorescence microplate reader-based assays.