Amplite(TM)Luminometric Alkaline Phosphatase Assay Kit *Luminescence*

Alkaline phosphatase (ALP) (EC 3.1.3.1) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. An important use of alkaline phosphatase is as a label for enzyme immunoassays. Our Amplite(TM) Luminometric Alkaline Phosphatase Assay Kit uses D-luciferin phosphate as the luminogenic phosphatase substrate to quantify alkaline phosphatase activity in solutions and in cells. D-luciferin phosphate is not recognized by luciferase until its phosphate group is removed to give luciferin. The kit provides an optimized 'mix and read' assay protocol which is compatible with HTS liquid-handling instruments. This Amplite(TM) Luminometric Alkaline Phosphatase Assay Kit can be readily performed in a 96-well or 384-well microtiter-plate format. Its signal can be easily read by luminescence microplate readers. The high sensitivity makes the kit ideal for the assays that require low detection limit. Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications. It is widely used in various biological assays (in particular, immunoassays) and ELISA-based diagnostics. This Amplite(TM) Alkaline Phosphatase Assay Kit uses our propreitary luminogenic phosphatase substrate, to quantify alkaline phosphatase activity in solutions and in cells. The kit provides all the essential components with our optimized 'mix and read' assay protocol which is compatible with HTS liquid-handling instruments. This Amplite(TM) Alkaline Phosphatase Assay Kit can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Its signal can be easily read by luminescence microplate readers. Its high sensitivity makes the kit ideal for the assays that require low detection limit.