Amplite(TM) Fluorimetric Glycerol Assay Kit

Ex: 571 nm. Em: 585 nm. Glycerol is a precursor for the synthesis of triglycerides and phospholipids in liver and adipose tissue. When fasting, triglycerides stored in these lipid droplets can be hydrolyzed to generate free glycerol and fatty acids. The amount of free glycerol released to the bloodstream is proportional to the triglyceride/fatty acid cycling rate, which is important in the metabolic regulation and heat production. Amplite(TM) Fluorimetric Glycerol Assay Kit offers a sensitive fluorescence-based assay for measuring glycerol levels in biological samples. This assay is based on an enzyme coupled reaction of glycerol, in which the product hydrogen peroxide can be detected using our Amplite(TM) HRP substrate in HRP-coupled reactions. The fluorescence signal can be measured by a fluorescence microplate reader at Ex/Em= 540/590 nm. With this Fluorimetric Glycerol Assay Kit, we were able to detect as low as 0.015 mg/L (~0.16 µM) glycerol in a 100 µL reaction volume.