Amplite(TM) Fluorimetric Coenzyme A Quantitation Kit (Green Fluorescence)

Coenzyme A (CoA) is a universal and essential cofactor in all forms of cellular life acting as a principal acyl carrier in numerous biosynthetic, energy-yielding, and degradative pathways. It plays important roles in the synthesis and oxidation of fatty acids, pyruvate oxidation and the citric acid cycle. Measurement of CoA is one of the essential tasks for investigating biological processes and events in many biological systems. There are a few reagents or assay kits available for quantitating CoA content in biological systems. However, the existing commercial kits either lack sensitivity or have tedious procedures. Our Amplite(TM) Fluorimetric CoA Qutitation Assay Kit provides an ultrasensitive fluorimetric assay to quantitate CoA content by detection of ?SH group in CoA. Our proprietary fluorogenic CoA Green(TM) dye used in the kit becomes strongly fluorescent upon reacting with ?SH. The assay kit can detect as little as 4 picomole of CoA in a 100 µL assay volume (40 nM). It can be performed in a convenient 96-well or 384-well microtiter-plate format at Ex/Em = 490/520 nm, and easily adapted to automation without a separation step.