Amplite(TM) Fluorimetric beta-Galactosidase Assay Kit *Green Fluorescence*

Amplite(TM) Fluorimetric beta-Galactosidase Assay Kit *Green Fluorescence*
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
ABD-12601 1 kit (500 assays) -

3 - 8 Werktage*

346,00 €
 
E. coli beta-galactosidase is a 464 kD tetramer. Each unit of beta-galactosidase consists of five... mehr
Produktinformationen "Amplite(TM) Fluorimetric beta-Galactosidase Assay Kit *Green Fluorescence*"
E. coli beta-galactosidase is a 464 kD tetramer. Each unit of beta-galactosidase consists of five domains, the third of which is the active site. It is an essential enzyme in cells. Deficiencies in this enzyme can result in galactosialidosis or Morquio B syndrome. In E. coli, beta-galactosidase is produced by the activation of LacZ operon. Detection of LacZ expression has become routine to the point of detection of as few as 5 copies of beta-galactosidase per cell. This kit uses a fluorogenic galactosidase substrate that can sensitively distinguish LacZ vs. LacZ-cells. It can be used either for detecting galactosidase conjugates in ELISA type assay systems or for monitoring LacZ gene expression in cells. The galactosidase-cleaved product has Ex/Em = 490/520 nm that can be detected with most of fluorescence instruments equipped with a FITC filter set.
Schlagworte: EC=3.2.1.23
Hersteller: AAT Bioquest
Hersteller-Nr: 12601

Eigenschaften

Anwendung: Galactosidase assays
Format: Homog.

Datenbank Information

Handhabung & Sicherheit

Lagerung: -20°C
Versand: +20°C (International: +20°C)
Signalwort: Warning
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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