Amplite(TM)Fluorimetric Alkaline Phosphatase Assay Kit *Green Fluorescence*

Alkaline phosphatase (ALP) (EC 3.1.3.1) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. An important use of alkaline phosphatase is as a label for enzyme immunoassays. Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical as well as Northern, Southern and Western blot applications. It is widely used in various biological assays (in particular, immunoassays) and ELISA-based diagnostics. Our Amplite(TM) Fluorimetric Alkaline Phosphatase Assay Kit uses our FDP, a fluorogenic phosphatase substrate, to quantify the alkaline phosphatase activity in solutions, in cell extracts, and on solid surfaces (such as PVDF membranes). It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Its signal can be easily read by a fluorescence microplate reader at Ex/Em = 490/525 nm. The kit provides an optimized 'mix and read' assay protocol which is compatible with HTS liquid handling instruments.