Amplite(TM)Fluorimetric Alkaline Phosphatase Assay Kit *Blue Fluorescence*

This Amplite(TM) Fluorimetric Alkaline Phosphatase Assay Kit uses our MUP Plus(TM)-based coumarin substrate. Similar to MUP, MUP Plus(TM) is sensitive to phosphatase-induced hydrolysis, giving the halogenated coumarin that possesses intense blue fluorescence. Its almost identical spectral properties to those of MUP enables MUP Plus(TM) substrates readily compatible with many fluorescence instrument systems equipped with MUP settings. Compared to MUP, MUP Plus(TM) gives the coumarin fluorophore that has substantially lower pKa, making the MUP Plus(TM) assay much less pH-dependent. Our Amplite(TM) Fluorimetric Alkaline Phosphatase Assay Kit uses our MUP Plus(TM), a fluorogenic phosphatase substrate, to quantify alkaline phosphatase activity in solutions, in cell extracts, and on solid surfaces (such as PVDF membranes). It can be performed in a convenient 96-well or 384-well microtiter-plate format. Its signal can be easily read by a fluorescence microplate reader at Ex/Em = 360/450 nm. The kit provides an optimized 'mix and read' assay protocol which is compatible with HTS liquid handling instruments. This Amplite(TM) Fluorimetric Alkaline Phosphatase Assay Kit uses our MUP Plus(TM)-based coumarin substrate. Similar to MUP, MUP Plus(TM) is sensitive to phosphatase-induced hydrolysis, giving the halogenated coumarin that possesses intense blue fluorescence. Its almost identical spectral properties to those of MUP enables MUP Plus(TM) substrates readily compatible with many fluorescence instrument systems equipped with MUP settings. Compared to MUP, MUP Plus(TM) gives the coumarin fluorophore that has substantially lower pKa, making the MUP Plus(TM) assay much less pH-dependent.