Amplite(TM) Colorimetric Glycerol Assay Kit

Ex: 575 nm. Glycerol is a precursor for synthesis of triglycerides and phospholipids in the liver and adipose tissue. When fasting, triglycerides stored in these lipid droplets can be hydrolyzed to generate free glycerol and fatty acids. The amount of free glycerol released to the bloodstream is proportional to the triglyceride/fatty acid cycling rate, which is important in the metabolic regulation and heat production. Amplite(TM) Colorimetric Glycerol Assay Kit offers a sensitive assay for measuring glycerol levels in biological samples. This assay is based on an enzyme coupled reaction of glycerol, in which the product hydrogen peroxide can be detected using our Amplite(TM) Red HRP substrate in the HRP-coupled reactions. The signal can be measured with an absorbance microplate reader using OD ratio of 570 nm/610 nm. With this Colorimetric Glycerol Assay Kit, we were able to detect as low as 0.15 µg/mL (~1.6 µM) glycerol in a 100 µL reaction volume.