Anti-phospho-S6 Ribosomal Protein(Ser235/236)

Anti-phospho-S6 Ribosomal Protein(Ser235/236)
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
R2031-29C.20 20 µl - -

1 - 19 Werktage

357,00 €
R2031-29C.100 100 µl - -

1 - 19 Werktage

650,00 €
 
To effectively promote growth and cell division in a sustained manner, growth factors and... mehr
Produktinformationen "Anti-phospho-S6 Ribosomal Protein(Ser235/236)"
To effectively promote growth and cell division in a sustained manner, growth factors and mitogens must upregulate translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase, which in turn phosphorylates the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation, particularly of mRNAs with an oligopyrimidine tract in their 5' untranslated regions (2). This group of mRNAs (5'TOP) encodes proteins involved in cell cycle progression and proteins that are part of the translational machinery, such as ribosomal proteins and elongation factors (2,3). The main in vivo S6 ribosomal protein phosphorylation sites, including Ser235, Ser236, Ser240 and Ser244, are located within a small 19 amino acid region in the S6 carboxy terminus (4,5). Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:2000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight., Immunohistochemistry (paraffin/frozen): 1:400, Immunofluorescence (IF-IC): 1:100, Flow Cytometry: 1:25, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Hersteller: United States Biological
Hersteller-Nr: R2031-29C

Eigenschaften

Anwendung: FC, IF, IHC, WB
Antikörper-Typ: Monoclonal
Klon: 8A30(D57.2.2E)
Konjugat: No
Wirt: Rabbit
Reaktivität: Human, Monkey, Mouse, Rat
Immunogen: Synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6.

Datenbank Information

Handhabung & Sicherheit

Lagerung: -20°C
Versand: +4°C (International: -20°C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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