Anti-phospho-MAP4K1 (Ser171) (Mitogen-activated Protein Kinase Kinase Kinase Kinase 1, MAPK/ERK Kina

Anti-phospho-MAP4K1 (Ser171) (Mitogen-activated Protein Kinase Kinase Kinase Kinase 1, MAPK/ERK Kina
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
M3949-71A-APC.200 200 µl - -

3 - 19 Werktage*

993,00 €
 
The c-Jun amino-terminal kinases (JNKs)/stress-activated protein kinases (SAPKs) play a crucial... mehr
Produktinformationen "Anti-phospho-MAP4K1 (Ser171) (Mitogen-activated Protein Kinase Kinase Kinase Kinase 1, MAPK/ERK Kina"
The c-Jun amino-terminal kinases (JNKs)/stress-activated protein kinases (SAPKs) play a crucial role in stress responses in mammalian cells. The mechanism underlying this pathway in the hematopoietic system is unclear, but it is a key in understanding the molecular basis of blood cell differentiation. We have cloned a novel protein kinase, termed hematopoietic progenitor kinase 1 (HPK1), that is expressed predominantly in hematopoietic cells, including early progenitor cells. HPK1 is related distantly to the p21(Cdc42/Rac1)-activated kinase (PAK) and yeast STE20 implicated in the mitogen-activated protein kinase (MAPK) cascade. Expression of HPK1 activates JNK1 specifically, and it elevates strongly AP-1-mediated transcriptional activity in vivo. HPK1 binds and phosphorylates MEKK1 directly, whereas JNK1 activation by HPK1 is inhibited by a dominant-negative MEKK1 or MKK4/SEK mutant. Interestingly, unlike PAK65, HPK1 does not contain the small GTPase Rac1/Cdc42-binding domain and does not bind to either Rac1 or Cdc42, suggesting that HPK1. activation is Rac1/Cdc42-independent. These results indicate that HPK1 is a novel functional activator of the JNK/SAPK signaling pathway. Applications: Suitable for use in FLISA and Dot Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap., , Note: Applications are based on unconjugated antibody.
Schlagworte: Anti-phospho-HPK1, Anti-phospho-MAP4K1, Anti-phospho-MEKKK 1, EC=2.7.11.1, Anti-phospho-MEK kinase kinase 1, Anti-phospho-Hematopoietic progenitor kinase, Anti-phospho-MAPK/ERK kinase kinase kinase 1
Hersteller: United States Biological
Hersteller-Nr: M3949-71A-APC

Eigenschaften

Anwendung: FLISA, Dot Blot
Antikörper-Typ: Polyclonal
Konjugat: APC
Wirt: Rabbit
Spezies-Reaktivität: human
Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser171 of human MAP4K1 (KLH).
Format: Affinity Purified

Handhabung & Sicherheit

Lagerung: +4°C
Versand: +4°C (International: +4°C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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