Anti-phospho-FADD (Ser194) (Fas-Associated Death Domain Protein, MORT1)

Fas-associated death domain (FADD or Mort 1) functions as an important adaptor in coupling death signaling from membrane receptors, such as the Fas ligand and TNF family (DR3, DR4 and DR5), to caspase- 8 (1,2). FADD has a C-terminal death domain interacting with the cytoplasmic tail of the membrane receptor and an N-terminal death effector domain interacting with caspase-8. Clustering of the receptors upon stimulation brings FADD together further to oligomerize caspase-8 and activate the caspase signaling pathway. Human FADD is phosphorylated mainly at Ser194 while mouse FADD is phosphorylated at Ser191. In both cases, the phosphorylation is cell cycle dependent (3) and may be related to its regulatory role in embryonic development and the cell cycle process (4,5), Applications: Suitable for use in ELISA, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight., Immunocytochemistry (ABC): 1:200, Optimal dilutions to be determined by the researcher., , Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.