Anti-Histone H3K9Me2 (Clone 9D8)

Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei. It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.{57056, 53978} Dimethylation of histone H3 at lysine 9 (H3K9Me2) is associated with transcriptional repression. Heterochromatin protein 1-alpha (HP1-alpha), HP1-beta, and HP1-gamma selectively bind histone H3 N-terminal peptides containing dimethylated lysine 9 over unmodified lysine 9. High nuclear levels of H3K9Me2 in tumor tissue are associated with decreased disease-specific and disease-free survival in patients with oral and oropharyngeal squamous cell carcinoma. Cayman's Histone H3K9Me2 Monoclonal Antibody (Clone 9D8) can be used for ELISA and flow cytometry (FC) applications.Synonyms: Dimethylated Histone H3 Lysine 9, Histone H3 containing dimethylated lysine 9. Immunogen: Synthetic peptide from the N-terminal region of human H3K9Me2 conjugated to KLH. Clone Designation: 9D8. Formulation: (Request formulation change), 100 µg of protein G-purified antibody. Storage Buffer: PBS, pH 7.2, with 50% glycerol, 0.1% BSA, and 0.02% sodium azide. Host: Mouse. Isotype: IgG2bkappa. Applications: ELISA, FC. Cross Reactivity: (+) H3K9Me3 (~50%), (-) H3K4Me2, (-) H3K27Me2, (-) H3K36Me2, (-) H3K4Me. UniProt Accession #: P68431.