Anti-c-myc (Myc, myc-1) (AP)

The c-myc gene product, is involved in the regulation of the cell cycle and cell growth. p62c-myc is primarily located to the cell nucleus, but has also been shown to localized to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers. Applications: Suitable for use in Immunohistochemistry, ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:500 -1:5000, Western Blot: 1:100 -1:500, detects a band of ~62kD in Colo 320HSR cell lysates. , Immunohistochemistry (frozen and paraffin): Does not require protein digestion pre-treatment of paraffin sections prior to staining. Does not require antigen retrieval using heat treatment prior to staining of paraffin sections., Optimal dilution determined by the researcher. Hybridoma: Spleen cells from immunized Balb/c mice were fused with cells of themyeloma cell line. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Protect from light.