Anti-Akt, pan (Rac PKa, PKBa) (Agarose)

Anti-Akt, pan (Rac PKa, PKBa) (Agarose)
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
A1124-12F.400 400 µl - -

3 - 19 Werktage*

688,00 €
 
Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis... mehr
Produktinformationen "Anti-Akt, pan (Rac PKa, PKBa) (Agarose)"
Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9) and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3alpha and beta (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3beta mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip (15) and p21 Waf1/CIP1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18). Inhibition of mTOR stops the protein synthesis machinery by inactivating p70 S6 kinase and activating the eukaryotic initiation factor 4E binding protein 1 (4E-BP1), an inhibitor of translation (18,19). Applications: Suitable for use in Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunoprecipitation: 1:20, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Schlagworte: Anti-AKT1, EC=2.7.11.1, Anti-PKB alpha, Anti-RAC-PK-alpha, Anti-Protein kinase B, Anti-Proto-oncogene c-Akt, Anti-Protein kinase B alpha, Anti-RAC-alpha serine/threonine-protein kinase
Hersteller: United States Biological
Hersteller-Nr: A1124-12F

Eigenschaften

Anwendung: IP
Antikörper-Typ: Monoclonal
Klon: 10g146(C67E7)
Konjugat: Agarose
Wirt: Rabbit
Spezies-Reaktivität: human, monkey, mouse, rat
Immunogen: Synthetic peptide corresponding to residues in the carboxy-terminal sequence of mouse Akt.
Format: Purified

Handhabung & Sicherheit

Lagerung: -20°C
Versand: +4°C (International: +4°C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
Hier kriegen Sie ein Zertifikat
oder , um Analysenzertifikate anzufordern.
Bewertungen lesen, schreiben und diskutieren... mehr
Kundenbewertungen für "Anti-Akt, pan (Rac PKa, PKBa) (Agarose)"
Bewertung schreiben
oder , um eine Produktbewertung abzugeben.
Zuletzt angesehen