Anti-Urokinase Plasminogen Activator Receptor (uPAR, CD87, uPAR-1, PLAUR (plasminogen activator rece

CD87, is a glycosyl phosphatidylinositol-linked receptor which serves to localize and enhance the proteolytic activity of the urokinase-type plasminogen activator (uPA). The, uPA/uPAR interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion and chemotaxis. Applications: Suitable for use in ELISA, Western Blot and Flow Cytometry., Other applications have not been tested. Recommended Dilutions: Blockade of Receptor-Ligand Interaction: In a functional ELISA, 0.5-2.5ug/ml of this antibody will block 50% of the binding of 50ng/ml of recombinant human u-Plasminogen Activator/Urokinase to immobilized recombinant mouse uPAR -Fc chimera coated at 5ug/ml (100ul/well). At 25ug/ml, this antibody will block >90% of the binding. , ELISA (direct): 0.5-1.0ug/ml. The detction limit is ~1ng/well., Western Blot: 1-2ug/ml. The detection limit is 25ng/lane under non-reducing conditions. , Flow Cytometry: 25ug/ml. Add 10ul of the diluted solution to 1-2.5 x 10e5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10ul of a 25ug/ml solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome., Optimal dilutions should be determined by each laboratory for each application. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile buffer. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.