Anti-Urokinase Plasminogen Activator Receptor (uPAR, CD87)

Urokinase plasminogen activator receptor (uPAR), also known as CD87, is a glycolipid anchored cell surface protein consisting of three homologous domains. It binds uPA with high affinity and enhances and localizes uPA catalyzed plasminogen activation, and thereby plays a key role in cancer invasion and in several tissue remodelling processes. In addition uPAR also binds to vitronectin and may hereby have biological functions independent of its role in uPA mediated proteolysis. Elevated levels of uPAR are associated with poor prognosis in many types of cancer. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Flow Cytometry. Other applications have not been tested. Recommended Dilutions: ELISA: Use as a detection antibody., Immunohistochemistry: Frozen and paraffin embedded tissues, Western Blot: Use on non-reduced proteins, Optimal dilutions to be determined by the researcher. Hybridoma: X63-Ag 8.6.5.3 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.