Anti-Troponin C1 (Troponin C, slow skeletal and cardiac muscles, TNNC1)

Troponin C1(TNNC1) (troponin C type 1 (slow)), also called Troponin C, slow-twitch skeletal muscle or TNC is a protein that in humans is encoded by the TNNC1 gene. Using a , 'monochromosomal' hybrid panel, the TNNC1 gene is mapped to chromosome 3, subsequent analysis of the Genebridge 4 radiation hybrid panel localized the gene to 3p in a region consistent with the earlier assignment. Circular dichroism measurements revealed changes in the secondary structures of , the TNNC1 mutants A8V, C84Y, and D145E. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.1-0.5ug/ml, Immunohistochemistry (Paraffin): 0.5-1ug/ml. Boiling the paraffin sections in 10mM citrate buffer, pH 6.0 for 20 minutes is required for the staining of formalin/paraffin sections., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.