Anti-Tenascin C (Tenascin-C, TNC, TN-C, TN, Cytotactin, Glioma-associated Extracellular Matrix Antig

Tenascin is a high molecular weight, multifunctional, hexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. It has been described under a variety of names: cytotactin, hexabrachion protein, J1, myotendinous antigen (MI) and glioma mesenchymal extracellular matrix (GMEM). The tenascin molecule is a disulfide-linked hexamer. The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:1000-1:2000, Immunohistochemistry (Paraffin/frozen): Staining of paraffin sections may be enhanced using trypsin digestion or heat mediated antigen retrieval using sodium citrate buffer pH 6.0., Optimal dilutions to be determined by the researcher. Positive Control: Human tonsil, Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo, I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo, I1904-14C: IgG, Fc (HRP) Pab Gt xMo, I1904-19C: IgG, F(ab')2 (HRP) Pab Gt xMo, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.