Anti-TAR DNA Binding Protein 43 (TARDBP, TDP-43, TDP43)

HIV-1, the viral cause of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression, by the transactivator Tat, is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. TARDBP is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. Recently it has been shown that TDP-43 accumulates abnormally in post-mortem brain tissue from individuals diagnosed with both ALS (Amyotrophic Lateral Sclerosis) and FTD (Frontal Temporal Dementia). This observation provides a long sought connection between two disease that often over-lap clinically. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence/Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml, Immunofluorescence (IC): 1:100-1:1000. A nuclear signal is present in MCF-7 cells., Optimal dilutions to be determined by the researcher. Positive Control: Hela Whole Cell Extract, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.