Anti-Substance P Receptor, aa385-407 (NK1 Receptor, Neurokinin Receptor)

The tachykinins belong to an evolutionary conserved family of peptide neurotransmitters that share the C-terminal sequence Phe-X-Gly-Leu-Met-NH2 and have an established role in neurotransmission. The mammalian tachykinins include substance P, neurokinin A (NKA) and neurokinin B (NKB) which exert their effects by binding to specific receptors. Tachykinin peptides are important in the mediation of many physiological and pathological processes including inflammation, pain, migraine headache and allergy induced asthma. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (Paraformaldehyde-fixed cryostat or Vibratome sections): 1:100-1:1000 , Western Blot: 1:500. Detects truncated form of Substance P Receptor on 10ug of mouse brain lysates., Optimal dilutions to be determined by the researcher. Positive Control: Spinal Cord, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.